FastQCFastQC Report
Wed 1 May 2024
EGAF00000729906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27817522
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC434242015.610376797760777TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG12934964.649932513758774TruSeq Adapter, Index 3 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATATCGTATGC637990.22934825035817352TruSeq Adapter, Index 3 (98% over 50bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT307560.11056340676211202Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC297730.10702966281468204TruSeq Adapter, Index 3 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)