FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000729972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15406609
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA3359382.1804798187583003TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1435450.9317105405868352TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT412080.26746962942981156TruSeq Adapter, Index 15 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTC253750.16470204442781666TruSeq Adapter, Index 15 (97% over 34bp)
ACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGC221240.14360071057816812TruSeq Adapter, Index 15 (96% over 28bp)
CACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTG214040.13892739148504385TruSeq Adapter, Index 15 (96% over 29bp)
AGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTC206920.134305998159621TruSeq Adapter, Index 15 (96% over 31bp)
GAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGT170530.11068626457645546TruSeq Adapter, Index 15 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTA157250.10206658713802629TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)