FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000730056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16363580
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA7708574.71080900389768TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT2435231.4882012371375946TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTA528500.3229733346859306TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT282950.17291448448322433TruSeq Adapter, Index 15 (96% over 33bp)
ACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGC200380.12245486623342813TruSeq Adapter, Index 15 (96% over 28bp)
CACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTG193280.11811596239942604TruSeq Adapter, Index 15 (96% over 29bp)
AAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTC166920.1020070180241732TruSeq Adapter, Index 15 (97% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)