FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000730058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20383827
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG9281214.553222513122781TruSeq Adapter, Index 1 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC6927333.3984442666237307TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG1188350.5829866982289439TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC613880.3011603267629773TruSeq Adapter, Index 1 (98% over 50bp)
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA379830.18633890485824867TruSeq Adapter, Index 1 (100% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)