FastQCFastQC Report
Wed 1 May 2024
EGAF00000730060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27565182
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA20399797.400564233531997TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT5176811.878024966423222TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATATCGTA433510.15726723661755618TruSeq Adapter, Index 18 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)