FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000730066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24574134
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC18726717.620496413017036TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG12270004.993054892595604TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC966730.39339331347342693TruSeq Adapter, Index 4 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG824800.3356374633588309TruSeq Adapter, Index 4 (97% over 49bp)
CACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCT442930.18024236377973685TruSeq Adapter, Index 4 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)