FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000730070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19736199
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA6707613.3986331410622683TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2624521.3298001302074427TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTA647590.3281229582251375TruSeq Adapter, Index 13 (97% over 40bp)
CACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTG342690.17363525773123792TruSeq Adapter, Index 13 (96% over 29bp)
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC316550.16039055949932404TruSeq Adapter, Index 13 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT244770.12402084109508624TruSeq Adapter, Index 13 (97% over 40bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTC242970.12310881137750994TruSeq Adapter, Index 13 (97% over 34bp)
AGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCT226580.11480427411580112TruSeq Adapter, Index 13 (96% over 33bp)
ACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGC217010.10995531611735371TruSeq Adapter, Index 13 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)