FastQCFastQC Report
Wed 14 Sep 2022
EGAF00000730084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20931942
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG10974455.242920126570196TruSeq Adapter, Index 1 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC7543933.6040277581506768TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG1014040.48444621144086875TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC518100.24751645117304452TruSeq Adapter, Index 1 (98% over 50bp)
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA394150.18830073196266262TruSeq Adapter, Index 1 (100% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)