FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000730174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12601828
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG7143625.668717268637534TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC5745164.558989378366377TruSeq Adapter, Index 4 (100% over 50bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG2030281.6110995960268621Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG589650.46790830663614835TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC390530.3098994844240058TruSeq Adapter, Index 4 (98% over 50bp)
CACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCT344770.2735872922563298TruSeq Adapter, Index 4 (100% over 50bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG263880.20939819207181687TruSeq Adapter, Index 4 (97% over 49bp)
AAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT228210.18109277479426TruSeq Adapter, Index 4 (100% over 50bp)
GATCGAAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC219020.1738001820053408TruSeq Adapter, Index 4 (98% over 50bp)
AGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTG217930.1729352281272209TruSeq Adapter, Index 4 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTC190880.15147008830782327TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC140730.11167427455762767TruSeq Adapter, Index 4 (97% over 46bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)