FastQCFastQC Report
Fri 31 Mar 2023
EGAF00000730216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20920625
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10755135.140921937083619TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG10494945.016551847757894TruSeq Adapter, Index 5 (100% over 49bp)
CACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCT1251230.5980844262539957TruSeq Adapter, Index 5 (100% over 50bp)
ACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTT532950.25474860335195526TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATG475410.227244644937711TruSeq Adapter, Index 5 (97% over 49bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC448580.21441998028261583TruSeq Adapter, Index 5 (97% over 46bp)
AGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTG436020.2084163355540286TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC385860.18443999641502107TruSeq Adapter, Index 5 (98% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT318260.15212738625160577TruSeq Adapter, Index 5 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTC292370.13975203895677113TruSeq Adapter, Index 5 (100% over 50bp)
GCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGC266200.12724285244824185TruSeq Adapter, Index 5 (100% over 50bp)
CACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTG261230.12486720640516237TruSeq Adapter, Index 5 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)