FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000730439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18692549
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTA9325974.989137650515187TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT4022672.1520178976125726TruSeq Adapter, Index 7 (97% over 36bp)
CACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTG551580.2950801412905217TruSeq Adapter, Index 7 (96% over 25bp)
ACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGC391510.20944709038879608TruSeq Adapter, Index 7 (95% over 24bp)
AGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCT367020.19634561343131962TruSeq Adapter, Index 7 (96% over 29bp)
AGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTC257590.1378035708238614TruSeq Adapter, Index 7 (96% over 27bp)
GCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCT251390.1344867412143737TruSeq Adapter, Index 7 (96% over 26bp)
CACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCT188130.10064437974724581TruSeq Adapter, Index 25 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)