FastQCFastQC Report
Sun 5 May 2024
EGAF00000730463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23528849
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA3032871.2890005796713642TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT982980.41777649216925145TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTA276730.11761306301043455TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)