FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000730476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17237545
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC12584767.300784421447486Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1188460.6894601290380967Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG266780.15476681859278685Illumina Single End PCR Primer 1 (98% over 50bp)
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT182150.1056705000625089Illumina Single End PCR Primer 1 (100% over 43bp)
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC173280.10052475570042022Illumina Single End PCR Primer 1 (100% over 47bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)