FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000730491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22815412
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG8139773.5676629464328764TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC7353923.223224721955492TruSeq Adapter, Index 5 (100% over 50bp)
CACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCT1034980.45363195720506827TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATG532590.2334343118590188TruSeq Adapter, Index 5 (97% over 49bp)
ACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTT417060.18279748794367598TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC393090.1722914317742761TruSeq Adapter, Index 5 (98% over 50bp)
AGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTG373210.16357802348693068TruSeq Adapter, Index 5 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT306540.13435654810879594TruSeq Adapter, Index 5 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTC285950.12533194666833103TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC248310.10883432655084205TruSeq Adapter, Index 5 (97% over 46bp)
GCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGC245340.10753257491032817TruSeq Adapter, Index 5 (100% over 50bp)
CACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTG239240.10485894359479461TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)