FastQCFastQC Report
Sat 13 May 2023
EGAF00000730493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25658911
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC309924012.07861081867426TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG22372058.719017732280221TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC1359620.5298821918046327TruSeq Adapter, Index 9 (98% over 50bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT1220730.475752848591275TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATG1203030.46885466027767114TruSeq Adapter, Index 9 (97% over 49bp)
GAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT626410.24412961251551166TruSeq Adapter, Index 9 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC590070.22996689142419177TruSeq Adapter, Index 9 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT549190.21403480451684018TruSeq Adapter, Index 9 (100% over 50bp)
ACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTT486640.1896573085272403TruSeq Adapter, Index 9 (100% over 50bp)
AGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTG466740.18190171827635243TruSeq Adapter, Index 9 (100% over 50bp)
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC446380.1739668530749415TruSeq Adapter, Index 9 (100% over 37bp)
GCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGC373440.14554008157244086TruSeq Adapter, Index 9 (100% over 50bp)
CACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTG311960.1215795947068837TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC276320.10768968332288147TruSeq Adapter, Index 9 (97% over 46bp)
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG259250.10103702374586357TruSeq Adapter, Index 9 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)