FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000730667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20730366
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG5187832.5025269693742986TruSeq Adapter, Index 1 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC4002421.9307039730991724TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG472110.22773838146417677TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC361970.1746085910880686TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)