FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000730741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15842284
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC10940706.906011784664383TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2840691.7931063475443314TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC532780.33630251799551125TruSeq Adapter, Index 9 (98% over 50bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT165820.10466925097416509TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)