FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000730755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17668212
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA15530248.789933016425206TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT2542331.4389288514310334TruSeq Adapter, Index 27 (97% over 44bp)
AGATCGGAAGAGCACATGTCTGAACTCCAGTCACATTCCTTTATCTCGTA367600.20805727257517626TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATATCGTA353930.20032021349981535TruSeq Adapter, Index 27 (97% over 43bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA301310.170537912947841TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTTTGAACTCCAGTCACATTCCTTTATCTCGTA238500.1349881923535896TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCTAGTCACATTCCTTTATCTCGTA211840.11989894619783824TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGTACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA207840.11763499328624764TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAATTCCAGTCACATTCCTTTATCTCGTA186580.10560208356114359TruSeq Adapter, Index 27 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)