FastQCFastQC Report
Mon 8 May 2023
EGAF00000730785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21972795
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA3612531.6440921603282606TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1172670.5336917765809949TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATATCGTA229470.10443368720274321TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)