FastQCFastQC Report
Mon 8 May 2023
EGAF00000730787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21639703
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG12474585.764672463388245TruSeq Adapter, Index 6 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC3654071.6885952639923016TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATG326760.15100022398643825TruSeq Adapter, Index 6 (97% over 49bp)

[WARN]Adapter Content

Adapter graph