FastQCFastQC Report
Tue 26 Sep 2023
EGAF00000730795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24777573
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG17696237.142035259062701TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC7601693.067971992252833TruSeq Adapter, Index 7 (100% over 50bp)
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA430900.17390726686588714TruSeq Adapter, Index 7 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCGTATG423870.17107002368633925TruSeq Adapter, Index 7 (97% over 49bp)
CACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCT253110.10215286218710766TruSeq Adapter, Index 7 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)