FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000730827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22992602
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA7368063.2045350935052936TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT2125450.9244060328622223TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT444870.19348397367118347TruSeq Adapter, Index 15 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTA355310.15453231435050283TruSeq Adapter, Index 15 (97% over 40bp)
AAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTC277840.12083886808461261TruSeq Adapter, Index 15 (97% over 34bp)
CACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTG277500.12069099443377483TruSeq Adapter, Index 15 (96% over 29bp)
AGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTC268060.11658532601051418TruSeq Adapter, Index 15 (96% over 31bp)
ACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGC264390.11498916042647109TruSeq Adapter, Index 15 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)