FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000730893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000730893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24612991
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA20636408.384352799706464TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT15346426.235089428993006TruSeq Adapter, Index 13 (97% over 40bp)
CACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTG3481111.4143384686566536TruSeq Adapter, Index 13 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCT2024350.8224721652073899TruSeq Adapter, Index 13 (96% over 33bp)
ACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGC1977470.8034253130795846TruSeq Adapter, Index 13 (96% over 28bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTC1747220.7098771538981183TruSeq Adapter, Index 13 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTA1680860.6829157821574793TruSeq Adapter, Index 13 (97% over 40bp)
GCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCT1601840.6508107852475142TruSeq Adapter, Index 13 (96% over 30bp)
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC1454100.5907855733583943TruSeq Adapter, Index 13 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT1041920.42332116401456454TruSeq Adapter, Index 13 (97% over 40bp)
CACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCT1028080.4176981172259803TruSeq Adapter, Index 13 (96% over 27bp)
CGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTG626290.25445505586866707RNA PCR Primer, Index 36 (100% over 29bp)
GAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTT527490.21431365249351453TruSeq Adapter, Index 13 (96% over 32bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGT428050.17391222383334068TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG426100.17311995929304164TruSeq Adapter, Index 13 (97% over 35bp)
ACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTT380460.15457690615496508RNA PCR Primer, Index 36 (96% over 29bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT338660.1375940047270159TruSeq Adapter, Index 13 (97% over 40bp)
TCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAA306050.12434490387616848RNA PCR Primer, Index 36 (96% over 30bp)
GTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGA296320.12039170696483006RNA PCR Primer, Index 36 (96% over 30bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT249570.10139767247304482Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)