FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000731160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000731160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27690514
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA3220761.163127560579049TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT996040.3597044099650877TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTA312710.1129303703065967TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)