FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000731166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000731166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20896432
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC7839333.7515160482899663TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG7008363.3538548590496218TruSeq Adapter, Index 5 (100% over 49bp)
CACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCT835600.39987687850251186TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATG474040.22685212480293288TruSeq Adapter, Index 5 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC382660.1831221712874236TruSeq Adapter, Index 5 (98% over 50bp)
ACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTT366270.17527872700947225TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC281070.13450621618082936TruSeq Adapter, Index 5 (97% over 46bp)
AGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTG275270.13173062272066352TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)