FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000731170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000731170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22877209
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT10148514.436078719217891TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA9257904.046778608352094TruSeq Adapter, Index 13 (97% over 40bp)
CACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTG965820.42217562465771064TruSeq Adapter, Index 13 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT685630.29970002022536923TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTA671240.29340991726744287TruSeq Adapter, Index 13 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCT586190.2562331794931803TruSeq Adapter, Index 13 (96% over 33bp)
ACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGC571610.24986002444616384TruSeq Adapter, Index 13 (96% over 28bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTC536360.23445167633866526TruSeq Adapter, Index 13 (97% over 34bp)
GCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCT466740.20401964243103257TruSeq Adapter, Index 13 (96% over 30bp)
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC430710.18827034364200634TruSeq Adapter, Index 13 (96% over 31bp)
CACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCT271590.11871640461037009TruSeq Adapter, Index 13 (96% over 27bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)