FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000731260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000731260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9922820
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG3548913.5765135314356202TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC3491913.5190701836776235TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCGTATG125200.12617380946142326TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCGTATGC111570.11243779490104629TruSeq Adapter, Index 7 (98% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTC103680.10448642623770259TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)