FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000731324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000731324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17877411
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG4592522.568895462547681TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1818141.0170040840924897TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATG246350.1377995952545925TruSeq Adapter, Index 12 (97% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)