FastQCFastQC Report
Wed 14 Sep 2022
EGAF00000731346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000731346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20716679
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTA10238404.942104861498312TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT3900801.882927278064211TruSeq Adapter, Index 7 (97% over 36bp)
CACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTG560090.27035703936909966TruSeq Adapter, Index 7 (96% over 25bp)
ACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGC372000.17956546027478631TruSeq Adapter, Index 7 (95% over 24bp)
AGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCT337480.16290255788584648TruSeq Adapter, Index 7 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATATCGTA246060.11877386332046753TruSeq Adapter, Index 7 (97% over 36bp)
AGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTC241680.11665962483658697TruSeq Adapter, Index 7 (96% over 27bp)
GCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCT226920.10953493076761966TruSeq Adapter, Index 7 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)