FastQCFastQC Report
Wed 1 May 2024
EGAF00000731359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000731359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24233943
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC14734876.080261061932844Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG572740.23633793312132492Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCC292100.1205334187672225Illumina Single End PCR Primer 1 (98% over 50bp)
CGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC256670.10591342894550838Illumina Single End PCR Primer 1 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)