FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000731374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000731374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15593167
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTA7213544.626090389463538TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT2238381.4354877363912026TruSeq Adapter, Index 7 (97% over 36bp)
CACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTG319180.20469222192002431TruSeq Adapter, Index 7 (96% over 25bp)
ACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGC192880.12369520572697001TruSeq Adapter, Index 7 (95% over 24bp)
AGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCT181240.11623039758376216TruSeq Adapter, Index 7 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)