FastQCFastQC Report
Mon 6 May 2024
EGAF00000731420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000731420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20625650
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA4905522.378358985050168TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT1504660.729509130621338TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTA414950.20118153852121023TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)