FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000731695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000731695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15867503
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA11995517.559796900621352TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT2093881.3196027125376941TruSeq Adapter, Index 22 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATATCGTA380420.23974786707146048TruSeq Adapter, Index 22 (97% over 40bp)

[WARN]Adapter Content

Adapter graph