FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000731699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000731699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21144862
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC461442521.82291376505555TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG338605816.013620708425528TruSeq Adapter, Index 4 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG2552181.2069977094199054TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC2279521.0780491260713831TruSeq Adapter, Index 4 (98% over 50bp)
CACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCT988340.46741378591167915TruSeq Adapter, Index 4 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT417130.19727250998374923TruSeq Adapter, Index 4 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTC358130.1693697504386645TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC332950.15746142017857576TruSeq Adapter, Index 4 (97% over 46bp)
AGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTG330890.15648718823513721TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCCATCTCGTATGC316360.14961554253700024TruSeq Adapter, Index 4 (98% over 50bp)
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC265930.125765777047871TruSeq Adapter, Index 4 (100% over 37bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT241470.11419795503985791Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGC227970.10781342531344021TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACTGACCAATCTCGTATGC219780.10394014394608014TruSeq Adapter, Index 4 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)