FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000732064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000732064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22870251
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA19225758.406444686593078TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT2618581.1449721299517002TruSeq Adapter, Index 27 (97% over 44bp)
AGATCGGAAGAGCACATGTCTGAACTCCAGTCACATTCCTTTATCTCGTA454250.19862046988465498TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATATCGTA431620.18872551945319707TruSeq Adapter, Index 27 (97% over 43bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA356760.15599304091590424TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTTTGAACTCCAGTCACATTCCTTTATCTCGTA292990.12810965651404524TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCTAGTCACATTCCTTTATCTCGTA257480.11258293579725033TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGTACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA250010.10931668393145313TruSeq Adapter, Index 27 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)