FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000732090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000732090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16742418
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA6645503.969259398493097TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT3110981.8581425932622158TruSeq Adapter, Index 25 (97% over 44bp)
CACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTG547520.32702564229372366TruSeq Adapter, Index 25 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)