FastQCFastQC Report
Fri 3 May 2024
EGAF00000732120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000732120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22078856
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA8478833.840248788252435TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT4166221.8869727670672791TruSeq Adapter, Index 25 (97% over 44bp)
CACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTG595040.26950671719585473TruSeq Adapter, Index 25 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)