FastQCFastQC Report
Wed 22 Feb 2023
EGAF00000732130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000732130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24228843
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA5751632.373877283368422TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1866130.7702101169255172TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT534280.2205140377524424TruSeq Adapter, Index 15 (96% over 33bp)
CACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTG342140.14121186059111449TruSeq Adapter, Index 15 (96% over 29bp)
AAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTC337120.13913994985233097TruSeq Adapter, Index 15 (97% over 34bp)
ACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGC334160.13791826543264984TruSeq Adapter, Index 15 (96% over 28bp)
AGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTC308950.12751331130421703TruSeq Adapter, Index 15 (96% over 31bp)
GAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGT267650.11046751179988247TruSeq Adapter, Index 15 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTA252290.10412796021667234TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)