FastQCFastQC Report
Wed 1 May 2024
EGAF00000732194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000732194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27008864
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG4479001.6583444605445086TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1804140.6679807044087451TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATG343520.12718787432155607TruSeq Adapter, Index 8 (97% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)