FastQCFastQC Report
Tue 19 Sep 2023
EGAF00000771566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33326983
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT22726096.819126111715542TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA22148906.645936117289705TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCATTAG620490.18618246962228774TruSeq Adapter, Index 14 (97% over 44bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT352110.10565312797741098TruSeq Adapter, Index 14 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCATAGA344020.1032256655215385TruSeq Adapter, Index 14 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)