FastQCFastQC Report
Sun 14 May 2023
EGAF00000771580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30473571
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1122538736.836467245666746TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA23151417.59720939826842TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT2912490.9557429288480829TruSeq Adapter, Index 19 (97% over 40bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1865390.6121337075986271Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1170640.3841492682298376TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTA704990.23134472819086416TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGACGATCTCGTAT570520.18721796667676394TruSeq Adapter, Index 19 (97% over 40bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT390190.1280420991684893TruSeq Adapter, Index 19 (97% over 40bp)
GATCTGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT367210.12050113851113807TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGGCTGAACTCCAGTCACGTGAAACGATCTCGTAT363790.11937885454907796TruSeq Adapter, Index 19 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)