FastQCFastQC Report
Thu 5 Jan 2023
EGAF00000771581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30473571
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG534244117.53139138173206Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC25341668.315946956134548Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG4181481.3721660648172807Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG2051780.6732981835309029Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGTGGTCGCCG1682770.5522063692502595Illumina Single End PCR Primer 1 (96% over 29bp)
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1560480.5120765137764787Illumina Single End PCR Primer 1 (100% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG706790.23193540396036944Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGTGGTCGCCG700480.22986475723504804Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGGCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG646850.21226590083584232Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGTGGGCGCCG550320.1805892719300931Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG548970.18014626510296414Illumina Single End PCR Primer 1 (100% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGTGGGCGCCG539080.1769008299027377Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG532210.17464641738245903Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG361450.11861097604872103Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG331180.10867777852487324Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGTGAAAGAGTGTAGATCTCGGTGGTCGCCG313120.10275133163750319Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)