FastQCFastQC Report
Thu 23 Mar 2023
EGAF00000771586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73861479
Sequences flagged as poor quality0
Sequence length100
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT779525910.553889666899305TruSeq Adapter, Index 13 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2098110.28406011203756154TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA1683250.22789280999910658TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTCAACAATCTCGTAT1266160.17142359145015224TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACAGTCAACAATCTCGTAT1264750.17123269356683204TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTCAACAATCTCGTAT1205540.1632163363530806TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACAGTCAACAATCTCGTAT1196870.16204251745351594TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTAACAATCTCGTAT1145060.15502803565577125TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1139970.15433890783584228TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACAGTCAACAATCTCGTAT1099000.1487920381339778TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1049600.14210384278928398TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT998810.13522745733266456TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTAT914300.12378576930472784TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCAACAATCTCGTAT813680.11016297141843044TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)