FastQCFastQC Report
Wed 22 Mar 2023
EGAF00000771590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70748014
Sequences flagged as poor quality0
Sequence length100
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT812958111.490896408767036TruSeq Adapter, Index 13 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2177180.3077372602996319TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA1732940.24494539168265556TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTCAACAATCTCGTAT1319260.18647307894748819TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACAGTCAACAATCTCGTAT1315870.18599391355353098TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTCAACAATCTCGTAT1267850.17920644387275664TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACAGTCAACAATCTCGTAT1193110.1686421897298771TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTAACAATCTCGTAT1188000.16791990797084425TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1165220.16470002960083094TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACAGTCAACAATCTCGTAT1152490.16290068580582345TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1088030.15378947598444248TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1087560.15372304302421833TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCAACAATCTCGTAT981260.1386978862756487TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTAT952400.13461861982443776TruSeq Adapter, Index 13 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)