FastQCFastQC Report
Tue 4 Apr 2023
EGAF00000771593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71046930
Sequences flagged as poor quality0
Sequence length100
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG28971724.077828556420383Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG3196010.44984491236989405Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1386120.19509921118336854Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGTGGTCGCCG1061010.1493393169838584Illumina Single End PCR Primer 1 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)