FastQCFastQC Report
Wed 22 Mar 2023
EGAF00000771628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70886504
Sequences flagged as poor quality0
Sequence length100
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT824269811.628021604789538TruSeq Adapter, Index 13 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2169320.3060272234613235TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA1716940.242209715970758TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACAGTCAACAATCTCGTAT1343180.18948317722087127TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTCAACAATCTCGTAT1337260.18864803940676778TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTCAACAATCTCGTAT1274260.17976059307424724TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTAACAATCTCGTAT1202440.16962890425517388TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACAGTCAACAATCTCGTAT1199720.1692451922865317TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1194110.16845378635120728TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACAGTCAACAATCTCGTAT1164270.1642442403422801TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1114760.15725983608953265TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCAACAATCTCGTAT1058370.14930486626904327TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1032720.14568640597651705TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTAT982690.13862864502388214TruSeq Adapter, Index 13 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)