FastQCFastQC Report
Mon 18 Sep 2023
EGAF00000771637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52891457
Sequences flagged as poor quality0
Sequence length100
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG26491985.008744606903153Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG2840160.5369789680779639Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1634730.3090725974896097Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTTTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG544870.10301663650521103Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGTGGTCGCCG540040.10210344555265324Illumina Single End PCR Primer 1 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)