FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000771640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31429838
Sequences flagged as poor quality0
Sequence length100
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC565356017.987875088633928TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2298210.7312191682311566TruSeq Adapter, Index 7 (100% over 49bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC867910.2761420532934341TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAGATCATCTCGTATGC848930.2701032057498992TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAGATCATCTCGTATGC825720.26271850335340574TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCAGATCATCTCGTATGC770480.24514284801595224TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAGATCATCTCGTATGC761980.24243841155019633TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCAGATCATCTCGTATGC745980.23734770761465585TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC740000.2354450570187476TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAGATCATCTCGTATGC738900.23509507112317918TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATTATCTCGTATGC731310.23268016844375716TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGATCATCTCGTATGC682090.21701989046205072TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC669280.21294414562365865TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCAGATCATCTCGTATGC655410.20853114164953698TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGC544290.1731762028172083TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)