FastQCFastQC Report
Mon 6 Feb 2023
EGAF00000771644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114701360
Sequences flagged as poor quality0
Sequence length100
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2564928022.361792397230513TruSeq Adapter, Index 7 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC14465751.2611663889599913TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG7390080.6442887861137828TruSeq Adapter, Index 7 (100% over 49bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC4638710.40441630334635964TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAGATCATCTCGTATGC4075740.3553349323844111TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAGATCATCTCGTATGC3778300.32940324334428117TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCAGATCATCTCGTATGC3701540.3227110820656355TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCAGATCATCTCGTATGC3538110.3084627767273204TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCAGATCATCTCGTATGC3517980.30670778445870217TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAGATCATCTCGTATGC3460800.3017226648402425TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAGATCATCTCGTATGC3427640.2988316790664034TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGATCATCTCGTATGC3341970.2913627179311562TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATTATCTCGTATGC3256450.28390683423457225TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC3255170.2837952400913119TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC3132060.2730621502656987TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)