FastQCFastQC Report
Mon 6 Feb 2023
EGAF00000771645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000771645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114701360
Sequences flagged as poor quality0
Sequence length100
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2440789421.279515779063125Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC7701600.6714480107297769Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT5790660.5048466731344772Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAAAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4204000.3665170142707985Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAACGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3899990.34001253341721493Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGAAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3416160.29783081909403686Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTAGATCTCGGTGGTCGCCG3366550.2935056742134531Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAAGGAAAGAGTGTAGATCTCGGTGGTCGCCG2863130.24961604640084478Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGAGAAAGAGTGTAGATCTCGGTGGTCGCCG2800260.24413485594242298Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGAAAAGAGTGTAGATCTCGGTGGTCGCCG2728730.23789866135850526Illumina Single End PCR Primer 1 (98% over 50bp)
GATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1961620.17101976820501516Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTATAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1956220.1705489804131355Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCATGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1914230.16688816941664858Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG1877720.1637051208459952Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCATCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1861090.16225526881285451Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCCG1612730.14060251770336463Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)